This book “Techniques in Molecular Biology and Germplasm Conservation” has been written to provide up-to-date and user friendly version of molecular techniques at much cheaper price to meet the practical requirements of the students, teachers and researchers dealing with Biotechnology, Microbiology, Biosciences, Genetics, Biochemistry, Plant Pathologists, and Plant Breeding subjects at both undergraduate and postgraduate levels. Various techniques have been described to facilitate decisions involving the right selection, precise procedure, appropriate use of specific methods with limitations for sterilisation, disinfection, isolation of microorganisms, preparation of culture media, DNA isolation and quantification, hybridisation and sequence based marker assisted selection, molecular diagnostics and germplasm conservation.
Additional Info
  • Publisher: Laxmi Publications
  • Language: English
  • ISBN : 978-93-83828-61-6
  • Chapter 1

    Sterilisation and Disinfection Techniques Price 2.99  |  2.99 Rewards Points

    The objective of this chapter is to help laboratory personnel dealing with various methods of sterilisation and disinfection in the areas of microbiology, biotechnology, pathology and for medical sciences. Various physical and chemical methods are being described to facilitate decisions involving the right selection, proper procedure and appropriate use of specific methods for sterilisation and disinfection.
  • Chapter 2

    Isolation of Microorganisms and Culture Media Price 2.99  |  2.99 Rewards Points

    Growing of any microorganisms in pure culture is essential for pathogenicity test, identification, characterisation and exploitation for variety of purposes. The most important step is the selection of tissue for isolation. Older lesions are often colonised by secondary or saprophytic organisms. in order to detect the primary pathogen (the causal agent) recently colonised tissue is selected i.e. at or near the active margins of the lesion to reduce the possibility of isolating saprophytic microorganism.
  • Chapter 3

    DNA Isolation and Quntification Price 2.99  |  2.99 Rewards Points

    The genomic DNA of fungi can be extracted from fresh cultures grown on various nutrient rich or selective broth culture media in conical flasks. The fungal mats are harvested by filtration using sterilized Whatman filter paper no. 1 fixed in funnel. The harvested mycelium is sufficiently dried at 40–50°C in an oven. Approximately 100 mg of dried fungal mycelium is crushed with a micropestle in 1.5 ml conical micro-centrifuge tube with liquid nitrogen.
  • Chapter 4

    Marker Assisted Selection Techniques Price 2.99  |  2.99 Rewards Points

    DNA based markers have been extensively used for a variety of purposes that includes molecular mapping, QTL (quantitative trait loci) analysis, molecular tagging, diagnostics, DNA fingerprinting, diversity analysis, preparation of high density genetic and physical maps for a large number of animal and plant genomes. These maps facilitate cloning of genes and determination of QTLs. The markers associated with the genes and or QTLs have also been practically used for MAS (marker assisted selection) of desirable plants at the seedling stage in segregating generation.
  • Chapter 5

    Molecular Diagnostics Techniques Price 2.99  |  2.99 Rewards Points

    Molecular diagnostic techniques have evolved in step with the discoveries of molecular biology over the past 25 years. These techniques have several advantages over classical techniques as they are more sensitive, specific and robust and are independent of environmental variations. Molecular diagnostics techniques are either nucleic acid based or antibodies based techniques.
  • Chapter 6

    Conservation Techniques of Biological Resources Price 2.99  |  2.99 Rewards Points

    The use of microorganisms and cell cultures is increasing world over with the advancement in biotechnology techniques to solve food, health, energy and environmental problems. Once microbial strains, newly isolated strains, genetically engineered strains, cell cultures and plant germplasm have been acquired, they have to be preserved systematically in a well organized manner. These genetic resources are collected and maintained throughout the world at regional, national and international level by the gene Banks.
  • Chapter 7

    Molecular Data Analyses Price 2.99  |  2.99 Rewards Points

    AMOVA (Analysis of molecular variance), a statistical model for establishment of molecular variation in a single biological species was developed by Excoffier et al. (1992). This program, which runs on Windows is called Arlequin and is freely available on Excoffier’s website. An additional statistical package, GenAlEx allows for complex genetic analyses to be employed and compared within the commonly used Microsoft Excel interface. This software allows for calculation of analyses such as AMOVA, as well as comparisons with other types of closely related statistics including F-statistics and Shannon’s index and more.

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